Brucella Broth (Becton Dickinson) 2.8 g Aqua dem. 80.0 ml Adjust with HCl to pH 5.0, make anoxic under a stream of N2/CO2, distribute in Hungate tubes to 8 ml and autoclave. Add aseptically to each tube: Fetal Bovine Serum (heat inactivated) 2.00 ml Vitox (Oxoid) 0.06 ml Skirrow supplement (Oxoid) 0.02 ml Amphotericin (10 mg/ml) 0.02 ml Add 1 ml of sterile air to each tube.
