L-Alanine 45.0 mg L-Phenylalanine 165.0 mg D-Maltose 2000.0 mg L-Arginine 174.0 mg L-Proline 115.0 mg D-Mannose 2000.0 mg L-Asparagine 150.0 mg L-Serine 525.0 mg D-Ribose 2000.0 mg L- Aspartic Ac 133.0 mg L-Threonine 119.0 mg D-Xylose 2000.0 mg L-Cysteine HCl 352.0 mg L-Tryptophan 102.0 mg L-Fucose 2000.0 mg L-Glutamic Ac 294.0 mg L-Tyrosine 90.0 mg Na-Citrate 200.0 mg L-Glutamine 680.0 mg L-Valine 117.0 mg N-acetylglucosamine 500.0 mg Glycine 75.0 mg KCl 968.0 mg Na-Pyruvate 550.0 mg L-Histidine 620.0 mg MgCl2 x 6H2O 203.3 mg Na-Fumarate 500.0 mg L-Isoleucine 131.0 mg NaH2PO4 x H2O 140.0 mg Adenine 1.40 mg L-Leucine 131.0 mg NH4Cl 1600.0 mg D-Arabinose 2000.0 mg L-Lysine 182.0 mg D-Galacturonic Ac 2000.0 mg D-Glucose 2000.0 mg L-Methionine 149.0 mg Hypoxanthine 1.40 mg Uracil 1.10 mg L-Ornithine HCl 168.0 mg Thymidine 2.40 mg ACES 1822.0 mg D-L-Carnatine 200.0 mg Putresine 5.00 mg Spermidine 5.00 mg *Phenol Red 10.0 mg Lactic Ac (30%) 1.40 mL Iso-Butyric Ac 10.0 μL 2-Methylbutyric Ac 10.0 μL Valeric Acid 10.0 μL Iso-Valeric Ac 10.0 μL CaCl2 Stock 1.0 mL Cu-Mn-Zn Stock 1.0 mL Se Stock 1.0 mL Ni-Sn-V-Mo Stock 1.0 mL Vitamin Solution 1.0 mL Hemin Solution 1.0 mL Lipoic Acid Soln 1.0 mL *Dissolve Phenol Red in a few drops of dilute NaOH. Then add to flask. Add the above ingredients to 500 mL of distilled water in a 1L volumetric flask.Adjust pH to 6.0. Prior to use, add the following solutions and bring the volume to 1.0 L with distilled water, adjust pH to 6.9, and filter sterilize. Bicarbonate Sol 1.0 mL Human Serum 10.0 mL Cholesterol Sol 1.0 mL DHNA Sol 1.0 mL Vitamin C Sol 1.0 mL Glutathione Sol 1.0 mL Yeast Extract Sol 20.0 mL Ferrous Sulphate Sol 1.0 mL Neopeptone Sol 10.0 mL
