培養基配方
Cultivation of Liberibacter sp.
ACES-buffer 10.0 g
Na2 α-ketoglutarate 2.0 g
KOH 3.75 g
destilled water 550 ml
adjust pH to 6.9, autoclave for 15 minutes and allow to cool. Add aseptically Fetal Bovine Serum 150 ml TNM-FH Medium (Sigma-Aldrich, T3285) 300 ml For preparation of agar plates add 15 g agar per liter of medium.
