(NH4)Cl 1.00 g NaCl 25.00 g MgCl2 x 6 H2O 7.00 g MgSO4 x 7 H2O 9.60 g CaCl2 x 2 H2O 0.50 g KCl 3.80 g K2HPO4 x 3 H2O 0.40 g Yeast extract 1.00 g Glucose 5.00 g Modified Wolin's mineral solution II 10.00 ml Distilled water 1000.00 ml 1. Final pH 7.0. 2. 3 g/l NaHCO3 are added from a filter-sterilized stock solution when the medium has cooled. Modified Wolin's mineral solution II (from medium 700) Nitrilotriacetic acid 1.500 g MgSO4 x 7 H2O 3.000 g MnSO4 x H2O 0.500 g NaCl 1.000 g FeSO4 x 7 H2O 0.100 g CoSO4 x 7 H2O 0.180 g CaCl2 x 2 H2O 0.100 g ZnSO4 x 7 H2O 0.180 g CuSO4 x 5 H2O 0.010 g AlK(SO4)2 x 12 H2O 0.020 g H3BO3 0.010 g Na2MoO4 x 2 H2O 0.010 g NiCl2 x 6 H2O 0.025 g Na2SeO3 x 5 H2O 0.300 mg Distilled water 1000.000 ml First dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH, then add minerals. Adjust final to pH 7.0 with KOH.
