培養基配方
K2HPO4 0.25 g
KH2PO4 0.25 g
(NH4)2SO4 0.50 g
MgSO4 x 7 H2O 25.00 mg
Trace element solution SL-10 1.00 ml
Selenite-tungstate solution 1.00 ml
Na-oxalate 5.00 g
Na-acetate 0.82 g
Yeast extract (OXOID) 1.00 g
Trypticase peptone (BD BBL) 1.00 g
Sodium resazurin (0.1% w/v) 0.50 ml
Na2CO3 4.00 g
L-Cysteine HCl x H2O 0.50 g
Distilled water 1000.00 ml
Dissolve ingredients except carbonate and cysteine, adjust pH to 6.8, bring medium to the boil and then cool to room temperature under 100% CO2 gas atmosphere. Add solid carbonate and cysteine, then equilibrate pH to 6.8. Dispense under 100% CO2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.
Trace element solution SL-10 (from medium 320)
HCl (25%) 10.00 ml
FeCl2 x 4 H2O 1.50 g
ZnCl2 70.00 mg
MnCl2 x 4 H2O 100.00 mg
H3BO3 6.00 mg
CoCl2 x 6 H2O 190.00 mg
CuCl2 x 2 H2O 2.00 mg
NiCl2 x 6 H2O 24.00 mg
Na2MoO4 x 2 H2O 36.00 mg
Distilled water 990.00 ml
First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally
make up to 1000.00 ml.
Selenite-tungstate solution (from medium 385)
NaOH 0.50 g
Na2SeO3 x 5 H2O 3.00 mg
Na2WO4 x 2 H2O 4.00 mg
Distilled water 1000.00 ml
