Solution A: (NH4)2SO4 3.00 g KCl 0.10 g K2HPO4 0.50 g MgSO4 x 7 H2O 0.50 g Ca(NO3)2 0.01 g Distilled water 700.00 ml Adjust pH to 2.0 - 2.2 with sulfuric acid. Solution B: FeSO4 x 7 H2O 44.20 g Distilled water 300.00 ml H2SO4, 10 N 1.00 ml (sterilize by filtration or handle under N2 before autoclaving to prevent oxidation of the iron) Solution C: Yeast extract (1% w/v in water) 20.00 ml After autoclaving, combine the three solutions. Medium pH 1.9 - 2.4.
