Solution A: KH2PO4 2.300 g Na2HPO4 x 2 H2O 2.900 g Distilled water 50.000 ml Solution B: NH4Cl 1.000 g MgSO4 x 7 H2O 0.500 g CaCl2 x 2 H2O 0.010 g MnCl2 x 4 H2O 0.005 g NaVO3 x H2O 0.005 g Trace element sol. SL-6 (see medium 27) 5.000 ml Distilled water 850.000 ml Agar (if necessary) 20.000 g Solution C: Ferric ammonium citrate 0.050 g Distilled water 20.000 ml Solution D: Yeast extract 1.000 g Na-acetate 1.000 g Na2-succinate 1.000 g DL-Malate 1.000 g Distilled water 30.000 ml pH 7.0 Solution E: Na-lactate 1.000 g Na-pyruvate 1.000 g D-Mannitol 1.000 g D-Glucose 2.000 g Distilled water 50.000 ml pH 7.0 filter-sterilized Standard vitamin solution: See next page Standard vitamin solution: Riboflavin 10.000 mg Thiamine-HCl x 2 H2O 50.000 mg Nicotinic acid 50.000 mg Pyridoxine-HCl 50.000 mg Ca-pantothenate 50.000 mg Biotin 0.100 mg Folic acid 0.200 mg Vitamin B12 1.000 mg Distilled water 100.000 ml H3P is a heterotrophic medium for growth, purity checking and isolation of a broad spectrum of aerobic bacteria (Ref. 3367). Solutions A, B, C and D are autoclaved separately for 15 min at 121?C, cooled down to 50?C and then mixed aseptically with filter-sterilized solution E (at 50?C) and 5.0 ml of standard vitamin solution. The final pH of this medium should be 6.8 without adjustment.
