培養基配方
Final pH: 6.9
Final volume: 1003 ml
Solution A 843.00 ml
Solution B 50.00 ml
Solution C 50.00 ml
Solution D 40.00 ml
Solution E 10.00 ml
Solution F 10.00 ml
Sparge solution A with 100% CO2 gas for 30 - 45 min to make it anoxic, then distribute under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave.Autoclave separately solutions B, C, E and F under 100% N2 gas, and solution D under 80% N2 and 20% CO2 gas atmosphere. To complete the medium appropriate amounts of solutions B to F are added to the sterile solution A in the sequence as indicated. Adjust pH of the complete medium to 6.9, if necessary.
Solution A
MgSO4 x 7 H2O 0.10 g
(NH4)2SO4 0.50 g
Fe(NH4)2(SO4)2 0.04 g
Na2MoO4 x 2 H2O (0.1% w/v) 2.40 ml
Na2SeO3 x 5 H2O (0.1% w/v) 0.15 ml
Tryptone 5.00 g
Yeast extract 5.00 g
Sodium resazurin (0.1% w/v) 0.50 ml
Distilled water 840.00 ml
Solution B
K2HPO4 7.00 g
KH2PO4 4.50 g
Distilled water 50.00 ml
Solution C
Glucose 18.00 g
Distilled water 50.00 ml
Solution D
Na2CO3 2.00 g
Distilled water 40.00 ml
Solution E
L-Cysteine HCl x H2O 0.30 g
Distilled water 10.00 ml
Solution F
Na2S x 9 H2O 0.30 g
Distilled water 10.00 ml
