K2HPO4 0.5 g Sodium glutamate 0.5 g Mannitol 10.0 g Yeast extract 1.0 g NaCl 0.05 g Solution A 10.0 ml Solution B 1.0 ml Solution C 1.0 ml Agar 20.0 g Distilled water 1000.0 ml adjust to pH 6.8 Solution A MgSO4 x 7 H2O 1.0 g Distilled water 100.0 ml Solution B CaCl2 x 2 H2O 5.28 g Distilled water 100.0 ml Solution C FeCl3 x 6 H2O 666 mg Distilled water 100.0 ml
