Bordet-Gengou Agar Base (BD 248200) 30.0 g Glycerol 10.0 ml Proteose Peptone 10.0 g DI Water 840 ml Adjust for pH 6.7 +/- 0.2. Autoclave at 121o C. Cool base medium to 45-50oC and add: Sterile Defibrinated Rabbit Blood 150 ml Broth Medium: Glycerol 10 ml Proteose Peptone 10.0 g Bordet-Gengou Broth Base (see below) 840 ml Adjust for pH 6.7 +/- 0.2. Autoclave at 121o C. Cool base medium to 45-50oC and add: Sterile Defibrinated Rabbit Blood 150 ml Bordet-Gengou Broth Base Potato 125 g NaCl 5.5 g DI Water 1000 ml Cut and boil 125 g of potato. Filter potato broth through cheese cloth and disgard leftover potato. Clarify broth further by running it through a filter again. Bring volume back to 1000 ml.
