Final pH: 7.0 Final volume: 1001 ml NaCl 15.00 g K2HPO4 1.00 g KH2PO4 0.15 g NH4Cl 0.50 g Na-acetate 0.82 g Trace elements solution (Pfennig, 1965) 1.00 ml MgSO4 x 7 H2O 1.00 g CaCl2 x 2 H2O 0.10 g Yeast extract 0.20 g D-Glucose 1.00 g Agar, for solid medium 15.00 g Distilled water 1000.00 ml Dissolve sodium chloride, hydrogenphosphates, ammonium chloride and acetate, adjust pH of solution to 7.0 - 7.2 and autoclave. After autoclaving add trace elements, magnesium sulfate, calcium chloride, yeast extract and glucose from sterile stock solutions. Adjust pH of complete medium to 7.0, if necessary. Trace elements solution (Pfennig, 1965) (from medium 1369) EDTA 5.00 g FeSO4 x 7 H2O 2.20 g ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.03 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.03 g NiCl2 x 6 H2O 0.03 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml pH 3.0-4.0
