NaCl 80.0 g Casamino Acids (BD 223050) 7.5 g Proteose peptone #3 5.0 g Yeast extract 1.0 g Sodium citrate 1.0 g MgSO4 . 7H2O 20.0 g K2HPO4 0.5 g Fe(NH4)2(SO4)2 . 6H2O 0.05 g Distilled water 1.0 L Agar (if desired) 15.0 g Autoclave at 121C for 15 minutes. For agar, set pH to 9.0. For broth, set pH to 8.0 to eliminate precipitation.