培養基配方
Yeast extract 1.0 g
Mannitol 10.0 g
Agar 15.0 g
Soil extract 200.0 ml
Distilled water 800.0 ml
Adjust pH to 7.0.
Soil extract:
Air-dried garden soil 80.0 g
Na2CO3 0.2 g
Distilled water 200.0 ml
Autoclave soil suspension for one hour at 121?C. To obtain a clear supernatant allow to settle and centrifuge. Adjust pH to 7.2.