培養(yǎng)基配方
Casamino acids (BD-Difco) 5.0 g
Yeast extract (BD-Difco) 5.0g
Sodium glutamate 1.0g
Trisodium citrate 3.0g
KCl 2.0g
MgSO4·7H2O 0.2g
FeCl2·4H2O 36.0mg
NaCl 100.0g
Bacto agar (BD-Difco) 20.0g
Distilled water 900.0ml
Adjust pH to 7.3.
After autoclaving, aseptically add 100 ml of 10% Na2CO3 solution to the medium. Check final pH to be about 9.5
