Nitrilotriacetic acid 0.1 g Micronutrient Solution (see below) 1.0 ml FeCl3 Solution (see below) 1.0 ml CaSO4 . 2H2O 0.06 g MgSO4 . 7H2O 0.10 g NaCl 0.008 g KNO3 0.103 g NaNO3 0.689 g Na2HPO4 0.111 g NH4Cl 0.2 g Yeast extract 0.5 g Glycyl-glycine 0.5 g Distilled water 1.0 L Prepare above medium and adjust pH to 8.2-8.4. Add 0.5 g sodium sulfide.Readjust pH to 8.2-8.4. For broth, filter-sterilize and dispense in tubes. For solid medium add 15 grams agar. FeCl3 Solution: FeCl3 0.2905 g Distilled water 1.0 L Micronutrient Solution: H2SO4 (concentrated) 0.5 ml MnSO4 . 7H2O 2.28 g ZnSO4 . 7H2O 0.50 g H3BO3 0.50 g CuSO4 . 2H2O 0.025 g Na2MoO4 . 2H2O 0.025 g CoCl2 . 6H2O 0.045 g Distilled water 1.0 L
