Yeast extract 8.0 g Peptone 15.0 g Glucose 10.0 g Acetic acid 3.0 ml Ethanol 5.0 ml Distilled water 992.0 ml The ethanol and acetic acid are sterilized separately by filtration (use filter resistant to these solvents), and added aseptically to the sterile medium. Adjust pH to 3,5 with concentrated HCl after autoclaving.
