Rehydrate and grow lyophilized cells from the ampoule in 5 ml liquid broth 545. Subsequent subculturing may be carried out in liquid medium 545 or on agar plates. 535. TRYPTONE SOYA AGAR (same as TRYPTICASE SOY BROTH AGAR) 545. TRYPTONE SOYA BROTH (TSB) Peptone from casein 17.0 g Peptone from soymeal 3.0 g D(+)-Glucose 2.5 g NaCl 5.0 g K2HPO4 2.5 g Agar if required 15.0 g Distilled water 1000.0 ml Adjust pH to 7.3. Medium 545 is the same as Caso Bouillon (Merck or Oxoid, Germany).
