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Malic acid 2.5 g
Yeast Extract (BD 212750) 1.0 g
(NH4)2SO4 1.25 g
MgSO4 . 7H2O 0.2 g
CaCl2 . 2H2O 0.07 g
Ferric citrate 0.01 g
EDTA 0.02 g
KH2PO4 0.6 g
K2HPO4 0.9 g
Trace Elements (see below) 1.0 ml
Vitamin Solution (see below) 7.5 ml
Distilled water to 1.0 L
Neutralize malic acid with NaOH and adjust the pH of the completed medium to 6.9. Autoclave at 121C for 15 minutes.
Trace Elements:
Ferric citrate 0.3 g
MnSO4 . H2O 0.002 g
H3BO3 0.001 g
CuSO4 . 5H2O 0.001 g
(NH4)6Mo7O24 . 4H2O 0.002 g
ZnSO4 0.001 g
EDTA 0.05 g
CaCl2 . 2H2O 0.02 g
Distilled water 100.0 ml
Vitamin Solution:
Nicotinic acid 0.2 g
Nicotinamide 0.2 g
Thiamine . HCl 0.4 g
Biotin 0.008 g
Distilled water 1.0 L

250g/瓶

常溫

3年