Final pH: 2.3 Final volume: 1001 ml Solution A 950.00 ml Solution B 50.00 ml Solution C 1.00 ml 1. Sterilize solution A and solution C by autoclaving at 121°C for 20 min and solution B by autoclaving at 112°C for 30 min. Prior to use mix solutions A and B and add trace elements solution. The final pH of the medium should be 2.3. 2. Note: At the beginning of growth incubate statically without shaking. For DSM 2391, DSM 14647, DSM 29468, DSM 27195, DSM 583: Adjust the pH of the complete medium to 1.8. Solution A (NH4)2SO4 132.00 mg MgCl2 x 6 H2O 53.00 mg KH2PO4 27.00 mg CaCl2 x 2 H2O 147.00 mg Distilled water 950.00 ml Adjust pH to 2.3 with 10 N H2SO4. Solution B (from medium 882) FeSO4 x 7 H2O 20.00 g H2SO4 (0.25 N) 50.00 ml The pH of this solution should be 1.2. Solution C (from medium 882) Trace element solution 1.00 ml Trace element solution (from medium 882) MnCl2 x 4 H2O 76.00 mg ZnCl2 68.00 mg CoCl2 x 6 H2O 64.00 mg H3BO3 31.00 mg Na2MoO4 10.00 mg CuCl2 x 2 H2O 67.00 mg Distilled water 1000.00 ml
