Sea water (filtered) 200.00 ml Mineral salt solution 150.00 ml NH4Cl 1.00 g Yeast extract 0.60 g K2HPO4 1.65 g Sodium resazurin (0.1% w/v) 0.50 ml L-Cysteine HCl x H2O 0.50 g Cellobiose 5.00 g Cellulose, Lens tissue or MN 301 (optional) 5.00 g Distilled water 650.00 ml 1. Dissolve ingredients (except cysteine and cellobiose) and sparge medium with 100% N2 gas for 30 - 45 m in to make it anoxic. Add cysteine, adjust pH to 7.2 and dispense under 100% N2 gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cellobiose from an anoxic stock solution sterilized by filtration. 2. Note: Some strains can be adapted to cellulose as substrate using 5.0 g/l cellulose (Lens tissue, OXOID; or cellulose powder MN 301, MACHEREY-NAGEL). If necessary adjust pH of completed medium to 7.2. Mineral salt solution (NH4)2SO4 6.00 g NaCl 6.00 g MgSO4 x 7 H2O 1.20 g CaCl2 x 2 H2O 0.80 g Distilled water 1000.00 ml
