Solution A: Na2S2O3 . 5H2O 5.0 g NH4Cl 1.0 g KNO3 2.0 g Distilled water 250.0 ml Solution B: KH2PO4 2.0 g Distilled water 250.0 ml Solution C: NaHCO3 2.0 g Distilled water 250.0 ml Solution D: MgSO4 . 7H2O 0.8 g FeSO4 . 7H2O (2%, w/v, in N HCl) 1.0 ml Trace Metals Solution (see below) 1.0 ml Distilled water 250.0 ml Sterilize the four solutions separately and combine aseptically for the completed medium. The pH of the final medium is 7.0. Trace Metal Solution: EDTA 50.0 g ZnSO4 22.0 g CaCl2 5.54 g MnCl2 5.06 g FeSO4 4.99 g Ammonium molybdate 1.10 g CuSO4 1.57 g CoCl2 1.61 g Distilled water 1.0 L Adjust pH to 6.0 with KOH.
