Final pH: 6.8 - 7.5 Final volume: 1000 ml Tryptone (BD BBL) 10.00 g Yeast extract 2.00 g Sodium resazurin (0.1% w/v) 0.50 ml FeSO4 x 7 H2O 0.20 g Sucrose 10.00 g Na2SO3 0.20 g Na2S2O3 x 5 H2O 0.08 g Distilled water 1000.00 ml Dissolve ingredients (except ferrous sulfate, sucrose, sulfite, and thiosulfate), adjust pH to 6.8 - 7.5, and sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic. Add ferrous sulfate, then dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add sucrose, sulfite, and thiosulfate from anoxic stock solutions prepared under 100% N2 gas and sterilized by filtration. The stock solution of sulfite should be freshly prepared. For DSM 8686, DSM 8690: Replace sucrose with 10.00 g/l D-xylose as substrate and adjust pH of medium to 5.2. For DSM 15493: Replace sucrose with 3.00 g/l D-maltose.
