NH4Cl 0.90 g NaCl 0.90 g MgCl2 x 6 H2O 0.40 g KH2PO4 0.75 g K2HPO4 1.50 g Trypticase peptone (BD BBL) 2.00 g Yeast extract (OXOID) 1.00 g Trace element solution SL-10 1.00 ml FeCl3 x 6 H2O (0.1% w/v in 0.2 N HCl) 2.50 ml Sodium resazurin (0.1% w/v) 0.50 ml L-Cysteine HCl x H2O 0.75 g Cellobiose 1.00 g Distilled water 1000.00 ml Dissolve ingredients except cysteine and cellobiose. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and adjust pH to 7.2. Distribute medium under same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Add cellobiose after autoclaving from an anoxic stock solution prepared under 100% N2 gas atmosphere and sterilized by filtration. Adjust pH of the complete medium to 7.2, if necessary. For DSM 8685, DSM 8691, DSM 8692, DSM 10170: Replace cellobiose with 5.00 g/l D-xylose added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Adjust pH of the complete medium to 5.2 - 5.4! For DSM 10319, DSM 11055, DSM 11056, DSM 11057, DSM 11426, DSM 22141, DSM 101588: Replace cellobiose with 5.00 g/l D-glucose added to the autoclaved medium from a sterile anoxic stock solution. For DSM 13528: Replace cellobiose with 5.00 g/l D-fructose added to the autoclaved medium from a sterile anoxic stock solution. Adjust pH of the complete medium to 6.0! For DSM 16488, DSM 24455: Replace cellobiose with 2.00 g/l D-glucose added to the autoclaved medium from a sterile anoxic stock solution. For DSM 22658: Replace cellobiose with 5.00 g/l D-fructose added to the autoclaved medium from a sterile anoxic stock solution. For DSM 24750: Replace cellobiose with 2.00 g/l Na-pyruvate added to the autoclaved medium from an anoxic stock solution sterilized by filtration. Trace element solution SL-10 (from medium 320) HCl (25%) 10.00 ml FeCl2 x 4 H2O 1.50 g ZnCl2 70.00 mg MnCl2 x 4 H2O 100.00 mg H3BO3 6.00 mg CoCl2 x 6 H2O 190.00 mg CuCl2 x 2 H2O 2.00 mg NiCl2 x 6 H2O 24.00 mg Na2MoO4 x 2 H2O 36.00 mg Distilled water 990.00 ml First dissolve FeCl2 in the HCl, then dilute in water, add and dissolve the other salts. Finally make up to 1000.00 ml.
