培養基配方
Peptone 10.0 g
Yeast extract 0.5 g
Cysteine-HCl x H2O 1.0 g
Agar 15.0 g
Calf serum 200.0 ml
Distilled water 800.0 ml
Adjust pH to 6.5.
Dissolve peptone, yeast extract and cysteine in 800 ml of distilled water. Adjust pH to 6.5 with NaOH. Add agar and dissolve by heating. Add 2 g/l activated charcoal to the agar base solution before autoclaving. After autoclaving, cool to 45°C and add serum.
