培養基配方
K2HPO4 0.22 g
KH2PO4 0.22 g
NaCl 0.45 g
(NH4)2SO4 0.45 g
CaCl2 x 2 H2O 0.06 g
MgSO4 x 7 H2O 0.09 g
Yeast extract 5.00 g
Xylan 5.00 g
Sodium resazurin (0.1% w/v) 0.50 ml
L-Cysteine HCl x H2O 0.15 g
Na2S x 9 H2O 0.15 g
Distilled water 1000.00 ml
Dissolve ingredients (except cysteine and sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas
atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. After sterilization add cysteine and sulfide from sterile anoxic stock solutions prepared under 100% N2 gas.
