Columbia agar base supplemented with 5% defibrinated sheep blood. Rehydrate pellets of freeze-dried ampoules in liquid medium 545, 215 or 1 (see below). ATTENTION: organisms, for which only M 693 is mentioned in the catalogue entry, do not grow in one of the media listed below! Those are meant to rehydrate the freezedried pellets, not for cultivation. 545. TRYPTONE SOYA BROTH (TSB) Peptone from casein 17.0 g Peptone from soymeal 3.0 g D(+)-Glucose 2.5 g NaCl 5.0 g K2HPO4 2.5 g Distilled water 1000.0 ml Adjust pH to 7.3. The same as Caso Bouillon (Merck or Oxoid, Germany). 215. BHI MEDIUM Brain Heart Infusion (Difco) 37.0 g Distilled water 1000.0 ml 1. NUTRIENT BROTH Peptone 5.0 g Meat extract 3.0 g Distilled water 1000.0 ml Adjust pH to 7.0.
