培養(yǎng)基配方
Mueller-Hinton broth 21.0 g
Yeast extract 5.0 g
Distilled water 1000.0 ml
For Haemophilus spp., add 15 mg NAD and 15 mg hemine per L (X + V factors).
NAD stock solution: dissolve 1 mg NAD per ml dist. water, filter sterilize.
Hemine stock solution: dissolve 50 mg hemine in 1 ml 1 M NaOH, make up to 25 ml with
dist. water, filter sterilize.
