Glucose 10.000 g K2HPO4 1.000 g NH4NO3 2.000 g MgSO4 x 7 H2O 0.200 g MnSO4 x 7 H2O 0.005 g FeSO4 x 7 H2O 0.005 g CaCl2 x 2 H2O 0.100 g Yeast extract 3.000 g Polypeptone (BBL) 0.300 g Agar 15.000 g Distilled water 1000.000 ml After sterilization add sterile 1 M Na-sesquicarbonate solution (1 ml in 10 ml) to achieve a pH of 9.7 (see medium 31). Na-sesquicarbonate solution: NaHCO3 4.200 g Na2CO2 (anhydrous) 5.300 g Distilled water 100.000 ml
