Yeast extract 0.30 g Na2-succinate 1.00 g (NH4)-acetate 0.50 g Fe(III) citrate solution (0.1% in H2O) 5.00 ml KH2PO4 0.50 g MgSO4 x 7 H2O 0.40 g NaCl 0.40 g NH4Cl 0.40 g CaCl2 x 2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.40 ml Trace element solution SL-6 (see below) 1.00 ml L-Cysteiniumchloride 0.30 g Resazurin(0,1%) 0.50 ml Distilled water 1000.00 ml Adjust pH to 6.8. Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121?C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp. Trace element solution SL-6: ZnSO4 x 7 H2O 0.10 g MnCl2 x 4 H2O 0.03 g H3BO3 0.30 g CoCl2 x 6 H2O 0.20 g CuCl2 x 2 H2O 0.01 g NiCl2 x 6 H2O 0.02 g Na2MoO4 x 2 H2O 0.03 g Distilled water 1000.00 ml
