Beef extract (Difco) 3.00 g Trypticase (BBL) 9.00 g Yeast extract 11.00 g NaCl 2.00 g Na2HPO4 0.40 g Na2CO3 0.25 g Haemin 5.00 mg Na-formate 2.00 g Na-fumarate 3.00 g Resazurin 1.00 mg Distilled water 1000.00 ml Adjust pH to 7.5. Prepare the medium anaerobically under 100% N2. Autoclave solutions of haemin (dissolved in 0.1 ml 1N NaOH, then diluted with water to 10 ml and neutralized), formate, and fumarate separately under nitrogen.
