培養基配方
KNO3 0.5 g
Sodium glycerophosphate 0.1 g
Trace Elements (see below)1.0 ml
Tris buffer 1.0 g
Tryptone (BD 211705) 2.0 g
Yeast extract 2.0 g
Agar (if needed) 15.0 g
Seawater 1.0 L
Adjust final pH to 7.0.
Autoclave at 121C for 15 minutes.
Trace Elements:
H3BO3 2.85 g
MnCl2 . 4H2O 1.8 g
FeSO4 1.36 g
Sodium tartrate 1.77 g
CuCl2 . 2H2O 26.9 mg
ZnCl2 20.8 mg
CoCl2 . 6H2O 40.4 mg
Na2MoO4 . 2H2O 25.2 mg
Distilled water 1.0 L
