酵母提取物 (Difco) 0.3 g Na2-succinate 1.0 g (NH4)-acetate 0.5 g Fe(III)citrate solution(0.1% in H2O) 5.0 ml KH2PO4 0.5 g MgSO4 x 7 H2O 0.4 g NaCl 0.4 g NH4Cl 0.4 g CaCl2·2 H2O 0.05 g Vitamin B12 solution (10 mg in 100 ml H2O) 0.4 mlg Trace element solution SL-10（見培養(yǎng)基1064） 1.0 ml L-Cysteiniumchloride 0.3 g Resazurin(0 1%) 0.5 ml 蒸餾水 1.0 L Adjust pH to 6.8
使用說明
Boil the medium for a few minute. Bubble the medium with nitrogen gas and fill 10 ml in 15 ml tubes with a rubber septum under a stream of nitrogen gas. Autoclave at 121C for 15 min. Sterile syringes are used to inoculate and remove samples. Incubate in the light using a tungsten lamp.
