NH4Cl 1.0 g KH2PO4 1.0 g Na2HPO4 x 7 H2O 0.1 g MgSO4 x 7 H2O 0.2 g CaCl2 x 2H2O 0.02 ml Yeast extract (Oxoid) 1.0 g Sigmacell alpha Type 50 (Sigma S5504) 5.0 g Cellobiose 0.5 g Agar 20.0 g Distilled water 1000.00 ml Adjust to pH 5.2 with H3PO4 prior to addition of carbon sources. Autoclave at 121°C for 15 minutes.
