KH2PO4 0.50 g K2HPO4 x 3 H2O 1.00 g Urea 2.00 g MgCl2 x 6 H2O 0.50 g CaCl2 x 2 H2O 0.05 g FeSO4 x 7 H2O (0.1% w/v in 0.1 N H2SO4) 1.25 ml Morpholinopropane sulfonic acid 10.00 g Sodium resazurin (0.1% w/v) 0.50 ml Yeast extract 6.00 g L-Cysteine HCl x H2O 1.00 g Cellobiose 5.00 g Distilled water 1000.00 ml Dissolve ingredients except cysteine and cellobiose and adjust pH to 7.2. Sparge medium with 100% N2 gas for 30 - 45 min to make it anoxic, then add cysteine and readjust pH to 7.2, if necessary. Dispense medium under the same gas atmosphere into anoxic Hungate-type tubes or serum vials and autoclave. Prior to inoculation add cellobiose from an anoxic stock solution prepared under 100% N2 gas and sterilized by filtration. For DSM 2360, DSM 103556, DSM 111537: Replace cellobiose with 5.00 g/l D-glucose added to the medium after autoclaving from an anoxic stock solution sterilized by filtration. For DSM 29353: With 1% cellobiose; anaerobic For DSM 101079: Add to the medium prior to autoclaving a stripe of lens cleaning tissue made of cellulose (e.g., from MACHEREY-NAGEL).
