Bacto peptone 5.00g Bacto yeast extract 1.00g Fe(III) citrate 0.10g NaCl 19.45g MgCl2 (anhydrous) 5.90g Na2SO4 3.24g CaCl2 1.80g KCl 0.55g NaHCO3 0.16g KBr 0.08g SrCl2 34.00mg H3BO3 22.00mg Na-silicate 4.00mg NaF 2.40mg (NH4)NO3 1.60mg Na2HPO4 8.00mg Distilled water 1000.00ml Final pH should be 7.6 ± 0.2 at 25?C. If using the complete medium from Difco add 37.40 g to 1 litre water. For DSM 11879 and DSM 16960 add to the medium after autoclaving 1.00 g/l Na2S2O3 x5 H2O from a stock solution sterilized by filtration. Check medium pH after autoclaving and adjust if necessary.
