培養基配方
Tryptone 20.00 g
Yeast extract 10.00 g
K2HPO4 1.04 g
KH2PO4 0.68 g
Sodium resazurin (0.1% w/v) 0.50 ml
Na2S x 9 H2O 0.15 g
Distilled water 1000.00 ml
Dissolve ingredients (except sulfide), adjust pH to 7.0, bring medium to the boil, then cool to room temperature under 100% N2 gas. Dispense under same gas atmosphere in culture vessels and autoclave. After sterilization add sulfide from a sterile anoxic stock solution prepared under 100% N2 gas.
