最新中文字幕-精品国产99-国产精品福利在线观看-粉嫩av一区二区三区-日韩精品久久久久-美丽的姑娘国语视频-亚洲欧洲色-国产不卡毛片-三级黄毛片-免费福利在线-日韩av三区-欧美成年人网站-欧洲美女与动交ccoo-成人涩涩软件-秋霞影院午夜老牛影院-婷婷久久伊人-呦呦视频在线观看-www.天天综合-日韩精品高清视频-国产欧美综合在线-欧美日韩一区二区三区在线视频-a猛片免费播放-91九色蝌蚪国产-国产欧美日韩专区-嫩模被强到高潮呻吟不断

(NH4)2SO4 2.00 g

KCl 0. 10 g

K2HPO4 0.50 g

MgSO4 x 7 H2O 0.50 g

Yeast extract 0.30 g

D-Glucose 1.00 g

Distilled water 1000.00 ml

Autoclave glucose and yeast extract separately as a 10% and 3%, resp., stock solution. Adjust pH of complete medium to 3.0 with 1 N H2SO4.

For DSM 11237, DSM 11244: Reduce amount of yeast extract to 0.1 g/l and adjust pH of medium to 3.5.

For DSM 11245: Replace yeast extract with 0.1 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 3.5.

For DSM 100189: Replace glucose with 1.8 g/l D-galactose and add 7.0 g/l FeSO4 x 7 H2O to the autoclaved medium from a 1 M stock solution (pH adjusted to 1.8 using 2 N H2SO4 and sterilized by filtration). Adjust pH of complete medium to 2.5.

For DSM 108313: Supplement medium with 1.0 g/l Trypticase Soy Broth (BD BBL) added from a sterile stock solution and adjust pH of medium to 4.0.

250g/瓶

常溫

3年