(NH4)2SO4 1.30 g KH2PO4 0.28 g MgSO4 x 7 H2O 0.25 g CaCl2 x 2 H2O 0.07 g FeCl3 x 6 H2O 0.02 g Allen's trace element solution 10.00 ml Yeast extract (OXOID) 1.00 g Distilled water 1000.00 ml Dissolve ingredients (except yeast extract or other substrates), adjust pH of the salt solution at room temperature to 2.0 using 10 N H2SO4 and autoclave. Yeast extract and other organic substrates are sterilized separately by autoclaving of a 10% (w/v) stock solution at neutral pH. For DSM 5348: Omit yeast extract and supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. For DSM 5389, DSM 7519, DSM 12421: Adjust pH of medium to 3.0 - 3.5. For DSM 6482, DSM 10039, DSM 104736: Reduce the amount of yeast extract to 0.20 g/l and supplement the medium with 5.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. For DSM 9789, DSM 9790: Use 2.00 g/l yeast extract and adjust pH of medium to 1.0 by using 300.00 ml 0.5 M H2SO4 and 700.00 ml distilled water for the dissolving of salts. For DSM 16993: Supplement medium with 1.00 g/l D-glucose and 1.00 g/l Casamino acids. Adjust pH of the completed medium to 3.0. For DSM 18247: Modified, with 0.5% yeast extract; solidified by 0.7% Gelrite; pH:9.0 For DSM 18786: Use only 0.10 g/l yeast extract and supplement medium with 10.00 g/l sulfide ore (e.g., chalcopyrite). Sterilize ore by heating at 150°C overnight. Adjust the pH of the medium to 0.8. For DSM 29038: Supplement medium with 3.00 g/l K2S4O6 added to the autoclaved medium from a stock solution sterilized by filtration. Adjust pH of completed medium to 2.5. For DSM 29099: Use only 0.20 g/l yeast extract and supplement medium with 1.00 g/l D-glucose and 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust pH of final medium to 2.5 - 3.0.
For DSM 45263: Adjust pH of medium to 7.5 For DSM 111728: Use only 0.50 g/l yeast extract and supplement medium with 10.00 g/l powdered sulfur. Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and add aseptically to the autoclaved medium. Adjust the pH of the final medium to 2.5. For DSM 112778: Supplement medium with 0.50 g/l powdered sulfur and 20.00 g/l sulfide ore (e.g., pyrite). Sterilize sulfur separately by steaming for 3 hours on each of 3 successive days (see medium 35) and ore by heating at 150°C overnight. Add sulfur and ore aseptically to the autoclaved medium. Adjust the pH of the medium to 3.0. Allen's trace element solution (from medium 88) MnCl2 x 4 H2O 180.00 mg Na2B4O7 x 10 H2O 450.00 mg ZnSO4 x 7 H2O 22.00 mg CuCl2 x 2 H2O 5.00 mg Na2MoO4 x 2 H2O 3.00 mg VOSO4 x 2 H2O 3.00 mg CoSO4 x 7 H2O 1.00 mg Distilled water 1000.00 ml Adjust pH of final solution to 2 with 1 N HCl.
