436. RUMINOCOCCUS ALBUS MEDIUM Tryptone 5.0 g Yeast extract 2.0 g Glucose 3.0 g Cellobiose 2.0 g Mineral solution 1 40.0 ml Mineral solution 2 40.0 ml Resazurin 1.0 mg Distilled water 920.0 ml Boil and cool under CO2. Under a gentle stream of CO2 add: Na2CO3 4.0 g Fatty acid mixture 1.0 ml Cysteine-HCl x H2O 500.0 mg Adjust pH to 7.0, distribute under N2 into appropriate vessels and sterilize. Mineral solution 1: K2HPO4 0.6 % Mineral solution 2: KH2PO4 0.6 % (NH4)2SO4 2.0 % NaCl 1.2 % MgSO4 x 7 H2O 0.25 % CaCl2 x 7 H2O 0.16 % Fatty acid mixture: Isobutyric acid 10.0 ml Isovaleric acid 10.0 ml 2-Methylbutyric acid 10.0 ml Distilled water 70.0 ml Inoculate under CO2 and incubate at 37?C.
