培養基配方
Mineral salt sol. ("Hutner/Cohen-Bazire") 20.00 ml
Peptone (Bacto) 0.25 g
Yeast extract (Bacto) 0.25 g
Agar (Bacto) 15.00 g
Distilled water 965.00 ml
Sterilize 20 min./121?C. After cooling to 60?C add to the medium:
Glucose sol. (2.5%, sterile-filtered) 10.00 ml
Vitamin sol. (double conc.) 5.00 ml
Adjust pH to 7.5 (the medium is only weakly buffered; one needs approx. 10 drops/l
medium of 6 N KOH).
Mineral salt solution:
Nitrilotriacetic acid (NTA) 10.00 g
MgSO4 x 7 H2O 29.70 g
CaCl2 x 2 H2O 3.34 g
Na2MoO4 x 2 H2O 12.67 mg
FeSO4 x 7 H2O 99.00 mg
Metall salt sol. "44" (see medium 590) 50.00 ml
Distilled water 900.00 ml
Dissolve NTA first by neutralizing with KOH, then add other salts. Adjust pH to 7.2 with
KOH or H2SO4. Adjust volume to 1000.0 ml with distilled water.
Vitamin solution (double conc.):
Biotin 4.00 mg
Folic acid 4.00 mg
Pyridoxine-HCl 20.00 mg
Riboflavine 10.00 mg
Thiamine-HCl x 2 H2O 10.00 mg
Nicotinamide 10.00 mg
D-Ca-pantothenate 10.00 mg
Vitamin B12 0.20 mg
p-Aminobenzoic acid 10.00 mg
Distilled water 1000.00 ml
Store in the dark and cold (5?C).
