培養基配方
Broth Medium
Brucella Broth (BD 211088) 28.0 g
DI Water 1000 ml
Autoclave at 121oC.
*This product is commercially available from BD.
To make medium from scratch, follow formulation below:
Tryptone (Trypticase) 10.0 g
Peptamin *(Peptone) 10.0 g
Dextrose 1.0 g
Yeast Extract 2.0 g
NaCl 5.0 g
Na2S2O5 0.1 g
DI Water 1000 ml
pH 7.0 +/- 0.2
*Peptic Digest of Animal Tissue