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cDNA 210
cDNA 210
規(guī)格:
價格:
編號:B243129
品牌:Mingzhoubio

標(biāo)準(zhǔn)菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
cDNA 210
商品貨號
B243129
Designations
cDNA 210
GenBank Number

X63546

培養(yǎng)基
Species
Homo sapiens, human
Depositors
M Hill
Applications
The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe.
Vector
Construct size (kb): 9.300000190734863
Insert
DNA: cDNA
Insert lengths(kb): 6.300000190734863
Tissue: DNA from ED1 cell line (tre-transfected NIH 3T3)
Gene product: tre-2 oncogene(tre-2 oncogene) [TRE2*]
Insert Size (kb)
6.300
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.7, 3.1, 2.4, 0.46; PstI--7.6, 1.45, 0.72; HindIII--5.2, 4.5; PvuII--2.6, 2.5, 1.10, 0.82, 0.74 (doublet), 0.49 (doublet); SalI--9.6.
The insert contains the following restriction sites (approximate kb from the 5' end): EcoRI--0.41, 3.96; PstI--0.67, 2.10; HindIII--1.92.
Contains two complete open reading frames, from nt 112-2472 and from nt 2460-4220 of the insert.
The 3.55 kb EcoRI fragment from the insert, which includes both coding regions, has been used as a probe. It detects mRNA of 8.2 kb in Ewing's sarcoma cells (the source of the transfected DNA) and other neoplasms.
Corresponds to nt 1586 - 7878 of the sequence record.
References

Nakamura T, et al. A novel transcriptional unit of the tre oncogene widely expressed in human cancer cells. Oncogene 7: 733-741, 1992. PubMed: 1565468

Nakamura T, et al. Nucleotide sequence of mouse L19 ribosomal protein cDNA isolated in screening with tre oncogene probes. DNA Cell Biol. 9: 697-703, 1990. PubMed: 1702292

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