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pcD-MCGF-b9 [moIL-3cDNA]
pcD-MCGF-b9 [moIL-3cDNA]
規格:
價格:
編號:B237766
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pcD-MCGF-b9 [moIL-3cDNA]
商品貨號
B237766
Designations
pcD-MCGF-b9 [moIL-3cDNA]
GenBank Number

K01668

培養基
Species
Mus musculus, mouse
Depositors
DNAX Research Institute of Molecular and Cellular Biology, Inc., EP Ching, DNAX Research Institute of Molecular and Cellular Biology, Inc.
Applications
contains sequence mast cell growth factor
Vector
Construct size (kb): 3.9499990940094
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pcDV1
Intact vector size: 3.000
Type of vector: plasmid
Cloning sites: SalI ClaI HindIII EcoRI KpnI
Polylinker sites:
Construction: pBR322, SV40
Host range: vertebrate cells; Escherichia coli
Features (with orientation and position when available):
enhancer: none
marker(s): ampR
promoter: none
replicon: pMB1
terminator: SV40 late
Cross references:
Insert
DNA: cDNA
DESCRIPTION OF INSERT COMPONENT:
Genome: mouse
Gene symbol: Il3
Other gene symbols: Mgf
Genomic copy number: unique
Gene name: interleukin 3 (colony-stimulating factor, multiple)
Contains complete coding sequence?: Y
Chromosome: 11; Localization: 11
Strain: C57BL/6
Tissue: T-cell line Cl.Ly1+ 2-/9, Con A-induced
Type of DNA: cDNA
Insert 5' end: Modification: GC
Insert 3' end: Modification: AT
Insert size (kb): 0.95
Cross references: DNA Seq. Acc.: K01668
Insert lengths(kb): 0.949999988079071
Tissue: T-cell line Cl.Ly1+ 2-/9, Con A-induced
Gene product: interleukin 3 (colony-stimulating factor, multiple) [Il3]
Target Gene: mast cell growth factor
Insert Size (kb)
0.950
Media
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: freeze-dried
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.2, 0.72, 0.45; HindIII--3.6, 0.72; XhoI--2.9, 1.1.
The insert contains the following restriction sites (approximate kb from the 5' end): BamHI--0.29; BglII--0.39; HindIII--0.10; XbaI--0.47.
Contains the complete coding sequence (nt 28-527).
Digestion with XhoI excises the insert plus flanking vector sequence.
Classification
Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod
References

Yokota T, et al. Isolation and characterization of a mouse cDNA clone that expresses mast-cell growth-factor activity in monkey cells. Proc. Natl. Acad. Sci. USA 81: 1070-1074, 1984. PubMed: 6322187

Yokota T, et al. cDNA clones coding for polypeptides exhibiting multi-lineage cellular growth factor activity. US Patent 4,695,542 dated Sep 22 1987

Disclosure
This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
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