天天综合永久,无码精品人妻一区二区三区湄公河,啪视频在线

pAT-A [K02403, SGC31623]

規格:

價格:

編號:B239815

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規格:

凍干粉

斜面

甘油

平板

加入購物車

在線咨詢收藏

產品名稱

pAT-A [K02403, SGC31623]

商品貨號

B239815

Designations

pAT-A [K02403, SGC31623]

GenBank Number

K02403

培養基

Species

Homo sapiens, human

Depositors

MC Carroll

Applications

The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI.

Vector

Construct size (kb): 8.5

Insert

DNA: cDNA

Insert lengths(kb): 5.400000095367432

Tissue: liver

Gene product: complement component 4A [C4A]

Alleles: D1, D2, A2, A1

Insert Size (kb)

5.400

Media

ATCC^® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information

Distributed: freeze-dried

Comments

Restriction digests of the clone give the following sizes (kb): BamHI--3.5 (doublet), 2.3; HindIII--9.5; HindIII/EcoRI--6.4, 2.9; HindIII/XhoI--4.1, 3.0, 2.2; PvuII--5.2, 2.3, 1.1, 0.5, 0.4; HindIII/SalI--6.3, 3.3.

Contains the entire coding sequence of pro C4A including approximately 60 bp 5' untranslated and approximately 140 bp 3' untranslated sequence plus poly(A).

The insert contains an internal BamHI site. The sequence is nearly identical to the C4B gene product.

The BglII RFLP is detected using the 400 bp BamHI/KpnI 5' fragment of pAT-A. The polymorphic site maps 2.5 kb upstream from C4A. The 4.2 kb allele segregates with F allele of factor B and the 6.6 kb TaqI fragment in C2.

The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI.

Enzyme(s) not detecting polymorphism: TaqI, BamHI, KpnI, HindIII, SalI, ClaI.

References

Schneider PM, et al. Polymorphism of the human complement C4 and steriod 21-hydroxylase genes. J. Clin. Invest. 78: 650-657, 1986. PubMed: 3018042

Belt KT, et al. The structural basis of the multiple forms of human complement component C4. Cell 36: 907-914, 1984. PubMed: 6546707

Palsdottir A, et al. BglII polymorphic site upstream to the human complement component C4A gene. Nucleic Acids Res. 15: 2395, 1987. PubMed: 2882470

McLean RH, et al. Restriction fragment analysis of duplication of the fourth component of complement (C4A). Genomics 2: 76-85, 1988. PubMed: 2838414

B179245：pAT-A [K02403, SGC31623]

王經理

手機號：17280875617

QQ：1438578920
胡經理

手機號：13345964880

QQ：2438244627
周經理

手機號：17757487661

QQ：1296385441
于經理

手機號：18067160830

QQ：2088210172
沈經理

手機號：19548299266

QQ：2662369050
李經理

手機號：13626845108

QQ：972239479