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pAT-A [K02403, SGC31623]
pAT-A [K02403, SGC31623]
規格:
價格:
編號:B179245
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
pAT-A [K02403, SGC31623]
商品貨號
B179245
Designations
pAT-A [K02403, SGC31623]
GenBank Number

K02403

培養基
Species
Homo sapiens, human
Applications
The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI.
Vector
Construct size (kb): 8.5
Insert
DNA: cDNA
Insert lengths(kb): 5.400000095367432
Tissue: liver
Gene product: complement component 4A [C4A]
Alleles: D1, D2, A2, A1
Insert Size (kb)
5.400
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information
Distributed: DNA (dried). Rehydrate with TE. (amount: 200 ng)
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--3.5 (doublet), 2.3; HindIII--9.5; HindIII/EcoRI--6.4, 2.9; HindIII/XhoI--4.1, 3.0, 2.2; PvuII--5.2, 2.3, 1.1, 0.5, 0.4; HindIII/SalI--6.3, 3.3.
Contains the entire coding sequence of pro C4A including approximately 60 bp 5' untranslated and approximately 140 bp 3' untranslated sequence plus poly(A).
The insert contains an internal BamHI site. The sequence is nearly identical to the C4B gene product.
The BglII RFLP is detected using the 400 bp BamHI/KpnI 5' fragment of pAT-A. The polymorphic site maps 2.5 kb upstream from C4A. The 4.2 kb allele segregates with F allele of factor B and the 6.6 kb TaqI fragment in C2.
The 2.4 kb BamHI/KpnI fragment can be used to detect C4A gene duplications in different haplotypes when the DNA is digested with TaqI.
Enzyme(s) not detecting polymorphism: TaqI, BamHI, KpnI, HindIII, SalI, ClaI.
References

Schneider PM, et al. Polymorphism of the human complement C4 and steriod 21-hydroxylase genes. J. Clin. Invest. 78: 650-657, 1986. PubMed: 3018042

Belt KT, et al. The structural basis of the multiple forms of human complement component C4. Cell 36: 907-914, 1984. PubMed: 6546707

Palsdottir A, et al. BglII polymorphic site upstream to the human complement component C4A gene. Nucleic Acids Res. 15: 2395, 1987. PubMed: 2882470

McLean RH, et al. Restriction fragment analysis of duplication of the fourth component of complement (C4A). Genomics 2: 76-85, 1988. PubMed: 2838414

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