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Human rhinovirus 31

規(guī)格:

價格:

編號:B236630

品牌:Mingzhoubio

標準菌株

定量菌液

DNA

RNA

規(guī)格:

凍干粉

斜面

甘油

平板

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產品名稱

Human rhinovirus 31

商品貨號

B236630

Classification

Picornaviridae, Rhinovirus, Human rhinovirus A

Agent

Human rhinovirus 31

培養(yǎng)基

Strain

140 F

Biosafety Level

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format

frozen

Storage Conditions

-70°C or colder

Images

Comments

This item has been treated with diethyl ether to remove Mycoplasma orale at the ATCC. The previous item number was VR-506™

Effect on Host

CPE, cell rounding and sloughing

Recommended Host

HeLa (ATCC^® CCL-2™)

Growth Conditions

Temperature: 33°C
Recommendations for Infection: For best results, infection should be performed on a 80-90% confluent, 18-48 hour old cellular monolayer. Prepare dilution of virus in minimum amount of volume (e.g. 1 mL per 25 cm²), optimized for propagation (e.g. MOI 0.005 - 0.01 recommended). Wash monolayer with PBS or serum free medium prior to inoculation. Adsorb virus dilution for 1-2 hours at 33°C in a humidified 5% CO₂ atmosphere, while rocking. End adsorption by adding virus growth medium.
Incubation: 1-7 days at 33°C in a humidified 5% CO₂ atmosphere while rocking, until CPE is progressed through 80% of the monolayer.

Name of Depositor

VV Hamparian and RM Conant

Source

Human throat washings

References

Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. I. HeLa cells for propagation and serologic procedures. J Immunol 100 (1): 107-113, 1968. PubMed: 4295059

Conant RM, and Hamparian VV. Rhinoviruses: Basis for a Numbering System. II. Serologic characterization of prototype strains. J Immunol 100 (1): 114-119, 1968. PubMed: 4295060

Connelly AP Jr., Hamre D. Virologic studies on acture respiratory disease in young adults. II. Characteristics and serolotic studies of three new Rhinoviruses. J. Lab. Clin. Med. 63: 30-43, 1964. PubMed: 14102901

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