Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Images
Comments
Virus primarily cell-associated. Cryopreserve virus in 7% DMSO (ATCC® 4-X™).
Effect on Host
CPE, rounding and clustering
Recommended Host
MRC-5 (ATCC® CCL-171™)
Growth Conditions
Temperature: 37°C Recommendations for Infection: This product is produced by co-cultivation of virus with fresh host cells. Prepare a bulk cell suspension the day of inoculation. Seed culture vessels at 1.3-1.5 x 104 cells per cm2. Calculate the volume of virus needed to achieve an optimal MOI (e.g. 0.1) and then dilute virus in virus growth medium to prepare the virus inoculum. Add virus inoculum to culture vessels. Incubate for 24 hours at 37°C in a humidified 5% CO2 atmosphere. Aspirate virus growth medium to remove any traces of DMSO and then add fresh virus growth medium to cultures. Continue incubation. Incubation: 14 days
Name of Depositor
ATCC®
Source
Clinical patient positive for CMV, Virginia, July 2011
