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The Vascular Smooth Muscle Cell Growth Kit contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete cell culture environment for smooth muscle cells isolated from large vessels. The final concentration for each component in complete vascular smooth muscle cell growth medium is as follows:

• rh FGF-basic: 5 ng/ml
• rh Insulin: 5 µg/ml rh EGF: 5 ng/ml L-glutamine: 10 mM
• Ascorbic acid: 50 µg/ml
• Fetal bovine serum: 5%

1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

Table 1. Vascular Smooth Muscle Cell Growth Kit Components  

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Component Volume Volume Sterility Tests Final Concentration Functional Tests rh FGF-basic C of A 0.5 mL 2026-06-16 明舟生物訂購流程 2026-05-29 明舟生物 618 菌株狂歡節(jié)｜三重福利送不停！ 2019-06-29 ATCC細胞運輸和處理 2019-06-28 其他海外品牌代理 2019-05-23 ATCC代理 2019-05-22 購買須知 B208382：Microvascular Endothelial Cell Growth Kit-VEGF B202603：Microvascular Endothelial Cell Growth Kit-BBE B186336：Primary Dermal Microvascular Endothelial Cells; Normal, Human, Neonatal (HDMVECn) B177343：Vascular Cell Basal Medium 王經(jīng)理 手機號：17280875617 QQ：1438578920 胡經(jīng)理 手機號：13345964880 QQ：2438244627 周經(jīng)理 手機號：17757487661 QQ：1296385441 于經(jīng)理 手機號：18067160830 QQ：2088210172 沈經(jīng)理 手機號：19548299266 QQ：2662369050 李經(jīng)理 手機號：13626845108 QQ：972239479 友情鏈接 中國林業(yè)微生物菌種保藏管理中心 中國工業(yè)微生物菌種保藏管理中心 中國普通微生物菌種保藏管理中心 美國國家生物技術(shù)信息中心(NCBI) SIB Bioinformatics Resource Portal https://www.weibo.com/p/10050 中國典型培養(yǎng)物保藏中心 ATCC菌株、細胞 菌株，慢病毒，細菌，真菌，質(zhì)粒載體 我的帳戶 登錄 注冊 購物車 我的訂單 用戶中心 客戶支持 在線客服 業(yè)務(wù)QQ 免費電話 在線支付 留言反饋 聯(lián)系我們 業(yè)務(wù)咨詢:0574-87157013 郵箱:mingzhoubio@163.com 浙江省寧波市高新區(qū)晶輝路866弄33號智造港E4幢3樓 Copyright ?2019 寧波明舟生物科技有限公司 mingzhoubio.com All Rights Reserved. 浙ICP備19018319號-1 <dfn id="kiscx"><table id="kiscx"><center id="kiscx"></center></table></dfn> <span id="kiscx"></span> <span id="kiscx"><table id="kiscx"></table></span>

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