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購物車 1 種商品 - 共0元
當(dāng)前位置: 首頁 > ATCC代理 > Microvascular Endothelial Cell Growth Kit-VEGF B208382
Microvascular Endothelial Cell Growth Kit-VEGF
Microvascular Endothelial Cell Growth Kit-VEGF
規(guī)格:
價格:
編號:B208382
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產(chǎn)品名稱
Microvascular Endothelial Cell Growth Kit-VEGF
商品貨號
B208382
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format
frozen 1 kit
培養(yǎng)基
Storage Conditions
-20°C (or -70°C for long-term storage)
Comments
The Microvascular Endothelial Cell Growth Kit-VEGF contains components that when added to Vascular Cell Basal Medium (ATCC PCS-100-030) create a complete, low serum culture environment for microvascular endothelial cells isolated from normal human tissue. The final concentration for each component in complete endothelial growth medium is as follows:
  • rh VEGF: 5 ng/ml
  • rh EGF: 5 ng/ml
  • rh FGF basic: 5 ng/ml
  • rh IGF-1: 15 ng/ml
  • L-glutamine: 10 mM
  • Heparin sulfate: 0.75 Units/ml
  • Hydrocortisone: 1 µg/ml
  • Ascorbic acid: 50 µg/ml
  • Fetal bovine serum: 5% 
Complete Growth Medium
  1. Obtain one growth kit from the freezer; make sure that the caps of all components are tight.
  2. Thaw the components of the growth kit just prior to adding them to the basal medium. It is necessary to warm the L-glutamine component in a 37°C water bath and shake to dissolve any precipitates, prior to adding to the basal medium.
  3. Obtain one bottle of Vascular Cell Basal Medium (475 mL) from cold storage.
  4. Decontaminate the external surfaces of all growth kit component vials and the basal medium bottle by spraying them with 70% ethanol.
  5. Using aseptic technique, and working in a laminar flow hood or biosafety cabinet transfer the volume of each growth kit component, as indicated in Table 1 or 2, to the bottle of basal medium using a separate sterile pipette for each transfer.

    6. Table 1. If using the Microvascular Endothelial Cell Growth Kit-BBE (ATCC® PCS-110-040), add the indicated volume for each component:  

        3. <address id="xcpab"></address>
        
        
            
        
                 
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
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                                            Sterility Tests                                        
        
                                        
        
                                            
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
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                                            Functional Tests                                        
        
                                        
        
                                            
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        Bovine Brain Extract (BBE)
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
         
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
                                                
        
                                    
        
                                                                                                                                                                    
                                    
        
                                        
        
                                            C of A                                        
        
                                        
        
                                            
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        1.0 mL
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
                
        
               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