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歷史搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
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S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
規格:
價格:
編號:B165651
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱
S49.1G.3 PHA.100/0 (replaces S49.1G3 PHA.12P)
商品貨號
B165651
Organism
Mus musculus, mouse
Cell Type
T lymphocyte
培養基
Product Format
frozen
Morphology
lymphoblast
Culture Properties
suspension
Biosafety Level
1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Disease
lymphoma
Strain
BALB/c
Applications
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Antigen Expression
H-2d; Thy-1.2; TL
Genes Expressed
H-2d; Thy-1.2; TL
Comments
This variant of S49.1G.3 (ATCC TIB-34) was selected for resistance to 5 mcg/ml phytohemagglutinin.
The cells are also resistant to 6-thioguanine and sensitive to thymidine, and hydrocortisone.
Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Name of Depositor
P Ralph
Deposited As
Mus musculus
References

Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

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